FIG 3.

E8 mutations increase HPV16 early and late transcripts in undifferentiated cells. Total RNA from HPV16 wt (#1 and #2), HPV16 E8− (E8− #1 and #2) and HPV16 E8 KWK mt (E8 KWK mt #1 and #2) cells grown in monolayer culture was analyzed by qRT-PCR for the expression of E6, E7, E1, E2, and L1 transcripts using PGK1 as a reference gene. Copy numbers are derived from plasmid dilutions of pBS 16 114b:1 and hPGK1 pEF-IRES-P plasmids measured in parallel. Data are derived from four independent RNA preparations for each cell line. Error bars indicate the standard errors of the means.