FIG 8.

E8^E2C transcripts are present in organotypic cultures. Quantitation of HPV16 E8^E2C transcripts in total RNA from undifferentiated (U) or differentiated (D) HPV16 wt cell lines by qPCR using PGK1 as a reference gene. Copy numbers are derived from plasmid dilutions of pSG 16 UTR E8^E2C and hPGK1 pEF-IRES-P plasmids measured in parallel. Data represent the averages for four (monolayer) or three (organotypic cultures) independent RNA preparations for each cell line, and error bars indicate the standard errors of means.