FIG 2.
Characterization of JEV replication in pericytes. (A) Pericytes were mock infected or infected with JEV (MOI, 20) over time. Cell viability was measured by MTS reduction and expressed as arbitrary units. n = 4. Pericytes (MOI, 20) and BHK21 cells (MOI, 5) were mock infected or infected with JEV over time. (B) Total RNAs were isolated and subjected to quantitative real-time RT-PCR for the measurement of JEV genome and β-actin. Relative JEV genome content was determined by the ΔΔCT method and expressed as arbitrary units. n = 4. (C) Total cellular proteins were isolated and subjected to Western blotting with antibodies against JEV NS3 and β-tubulin. Total cellular proteins obtained from mock-infected cells at the indicated times were used as a control. One representative blot of three independent experiments is shown. (D) The supernatants were collected and subjected to plaque assay for the determination of viral titers. n = 4.