FIG 1.

shRNA knockdown of cGAS, TBK1, and STING in MS1 endothelial cells. (A) RT-qPCR quantitation of mRNA levels in MS1 endothelial cells after knockdown and puromycin selection using shRNA lentiviral vectors targeting TBK1, STING, and cGAS. Samples were normalized to the cellular control TBP. One shSCR control sample was selected as representing a 100% standard. All qRT-PCR assays included biological triplicates as well as technical triplicates of each sample. (B) Western analysis of lysates harvested from wild-type (wt) or stable shRNA MS1 knockdown cell line pools at 6 or 24 h post-mock or -Ad5CiG infection using the indicated antibody. ns corresponds to a nonspecific band. M corresponds to mock treatment and AD to Ad5CiG infection. All experiments were carried out a minimum of three times; representative results are presented.