FIG 1.

BTV infects ovine splenocytes and thimocytes but not ovine HSCs. (A) Ovine BMCs were infected with BTV-8 at MOI of 0.1 PFU/cell. In parallel, cells isolated from ovine spleen and thymus were infected. The viral load in the supernatants of BMCs and spleen/thymus cells from 24 h to 7 days postinfection is shown as determined by plaque assay in Vero cells. (B) Histograms show the results of one representative experiment analyzing the expression of cell surface MHC-II and CD11c molecules on OvBM-DCs after 8 days in culture with GM-CSF. Shaded histograms indicate background staining with isotype control antibodies, black-lined histograms represent BTV-infected cultures, and dashed histograms represent mock-infected cultures.