FIG 2.

BTV replicates in HSCs and immature BM-DCs in the absence of IFN-I receptor. Supernatants of HSC and BM-DC cultures from IFNAR^−/− or C57BL/6 mice were taken at different times post-BTV infection, and viral titer was determined by plaque assay in Vero cells as indicated in Materials and Methods. (A) HSCs were infected with BTV-8 at MOI of 0.01 PFU/cell (t = 0). Supernatants were taken at days 1, 2, 3, 4, 6, 8, 10, 11, and 12 postculture. (B) BM-DC cultures were infected at day 6 postculture (immature phenotype) with BTV-8 at MOI of 0.01 PFU/cell (t = 6). Supernatants were taken at postculture days 7 (24 hpi), 8 (48 hpi), 10 (4 dpi), 11 (5 dpi), and 12 (6 dpi). (C) Mature BM-DC cultures, after 10 days in culture, were infected with BTV at MOI of 0.01 PFU/cell (t = 10). Supernatants were taken at day 11 postculture (24 hpi) and day 12 postculture (48 hpi). Each arrow indicates the viral input in each infection. The data are representative of the results of 4 independent experiments.