FIG 6.

BTV infection of HSC in vivo impairs BM-DC development in vitro. HSCs isolated from BTV-infected IFNAR^−/− mice at 2, 4, and 6 dpi were cultured in GM-CSF during 10 days and kept an additional 24 h in culture with LPS. (A) Dot plots show double staining for CD11c and CD11b molecules on mature BM-DCs from naive or BTV-infected IFNAR^−/− mice. The numbers in the upper right quadrant indicate the percentage of doubly positive CD11c and CD11b cells. (B) The MFI of surface molecules expressed on CD11c⁺ CD11b⁺ BM-DCs after LPS treatment is displayed. Each bar represents the MFI of a given surface molecule on CD11c⁺ CD11b⁺ BM-DCs from uninfected or BTV-infected mice HSCs isolated at 2, 4, and 6 dpi. Data are presented as means ± standard deviations (SD).