TABLE 6.
Sequence analysis of pertinent HA, M2, and PB2 amino acid residues
| Category | Amino acid(s)a |
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| HA receptor bindingc |
M2 adamantane antiviral resistanced |
PB2 mammal adaptatione |
||||||||||
| 190 | 222 | 226 | 228 | 26 | 27 | 30 | 31 | 34 | 271 | 627 | 701 | |
| Avian or α2,3 binding consensusb | E | – | Q | G | – | – | – | – | – | – | – | – |
| Human or α2,6 binding consensusb | D | – | L/Q | S/G | – | – | – | – | – | – | – | – |
| Adamantane-susceptible consensus | – | – | – | – | L | V | A | S | G | – | – | – |
| PB2 mammalian adaptation consensus | – | – | – | – | – | – | – | – | – | A | K | N |
| All avian viruses in this studyd | E | K/E | Q | G | L | V | A | S | G | T | E | D |
| Swine/MO/06b | E | K | L | G | L | I | A | S | G | A | E | D |
a
Sequences were obtained from in-house Sanger sequencing or GenBank. “–” indicates the residue does not pertain to the designated category.
c
HA analysis excludes Bwt/Alb/78, Mal/MN/00, Mal/MN/98.
d
M2 analysis excludes Bwt/Alb/78, Mal/MN/00, Dk/NJ/95.
e
PB2 analysis excludes Bwt/Alb/78, Mal/MN/00, Mal/MN/98, Mal/MN/08, Dk/NJ/95.