FIG 4.

Monoclonal antibodies precipitate two forms of NSP2. (A to C) Immunoprecipitations were performed with mock- and rotavirus-infected cell lysates using either MAb vNSP2_CT that detects viroplasmic NSP2 (A), MAb dNSP2_NT that detects dispersed NSP2 (B), or an isotype control MAb matched to vNSP2_CT isotype (C). The precipitated samples were analyzed by SDS-PAGE under nonreducing conditions, and Western blots were probed for NSP2 using the reciprocal MAb (dNSP2_NT or vNSP2_CT) or a guinea pig anti-NSP2 PAb. The NSP2 primary antibodies were detected with either goat anti-mouse or goat anti-guinea pig alkaline phosphatase secondary conjugates.