FIG 8.
Monoclonal antibodies to NSP2 coimmunoprecipitate VP1 and VP2. Rotavirus- and mock-infected cell lysates were prepared in RIPA buffer with protease inhibitors, and immunoprecipitations were performed with either MAb dNSP2NT or MAb vNSP2CT. (A and B) Proteins in the precipitated samples were analyzed by SDS-PAGE under nonreducing conditions, and Western blots were probed with PAb guinea pig anti-VP1, PAb guinea pig anti-VP2, and PAb guinea pig anti-NSP2 sera (A) or with PAb rabbit anti-NSP4 (B). The primary antibodies were detected with either goat anti-mouse or goat anti-guinea pig alkaline phosphatase secondary conjugates.