FIG 3.

Cell death in unsorted and sorted cell cultures. (A) Change in EGFP-positive cell numbers over time, scaled by the cell number on day 2. From day 2, cell numbers decline in all samples. Decline in the unsorted cell populations is slower than in the sorted. (B) In sorted cell cultures, the decline rate slows down in time significantly. (C) There is no difference in the rates of decay of cell numbers between EGFP-medium and EGFP-high cells in the same time period. (D) However, in the unsorted cell populations, EGFP⁺ cells are lost at the same rate between days 2 and 3 and between days 3 and 4.