TABLE 1.
Binding parameters of HTLV-2 proteinsa
| HTLV-2 protein (NaCl concn [mM]) | Mean Kd (nM) ± SD |
||
|---|---|---|---|
| ssDNA | SL1 | SL2 | |
| NC (50) | 215 ± 31 | 614 ± 120 | 419 ± 170 |
| MA (50) | 138 ± 3.0 | 341 ± 170 | 74 ± 18 |
| MA (100) | 945 ± 200 | — | 186 ± 80 |
| MA (150) | 6,200 ± 1,600 | — | 493 ± 97 |
| R47A/K51A MA (50) | 864 ± 320 | — | 633 ± 200 |
| R47A/K51A MA (100) | NB | — | NB |
| R47A/K51A MA (150) | NB | — | NB |
a
Shown are apparent equilibrium dissociation constants (Kd) obtained from FA measurements performed at various ionic strengths. NB indicates that no binding was detected with up to 7 μM protein. A dash indicates that the value was not determined.