FIG 6.

WSMV CP amino acids 6 to 27 and 85 to 100 are required for efficient virion assembly. (A) MIVA assay of wild-type virus and CP deletion mutants in agroinfiltrated Nicotiana benthamiana leaves at 7 dpa. Western blot analysis of partially purified virions from agroinfiltrated N. benthamiana leaves was performed with an anti-WSMV serum. Note that the deletion of amino acids 36 to 84 resulted in levels of accumulation of virions approximately similar to those for wild-type virus; in contrast, deletion of amino acids 85 to 100, 36 to 100, or 6 to 27 in the CP significantly affected virion assembly in WSMV. (B) Longer exposure of immunoblot lanes consisting of partially purified virions of WSMV CP mutants with deletions comprising amino acids 85 to 100, 36 to 100, or 6 to 27. The positions of CP and truncated CPs of 31 and 29 kDa are indicated with arrows, arrowheads, and an asterisk, respectively. The protein size markers used in SDS-PAGE are indicated.