FIG 2.

CLEM analysis of HCMV UL50 and UL53 null mutants. HFF cells were electroporated with UL50 null (50N) (A to D) or UL53 null (53N) (E to H) pBADGFP or the rescued derivative 50NR (I) or 53NR pBADGFP (J) and were seeded on gridded coverslips. Phase and fluorescence microscopy was performed on electroporated cells (A and E) on day 7 postelectroporation, and the cells were then processed for EM (B to D and F to J). The original GFP-positive cells (A and E) were traced back, and serial sections were scanned for nuclear and cytoplasmic nucleocapsids, for each sample. The insets in panels B, C, F, and G indicate regions magnified in the next panels (C, D, G, and H, respectively). Bars, 500 nm. N, nucleus; Cyt, cytoplasm; cB and cC, cytoplasmic B and C capsids, respectively. Arrows A, B, and C point to examples of nuclear A, B, and C capsids.