FIG 5.

Cellular distribution and nuclear rim localization of cellular kinases PKC and Cdk-1 during HCMV infection. (A) HFFs were mock infected (i to iv) or infected with HCMV (v to viii) at an MOI of 1. At 72 h p.i., cells were fixed and stained for lamin B (green) and PKC (red). The samples were visualized using confocal microscopy. (B) HFFs were mock infected (i) or infected with HCMV (ii and iii) at an MOI of 1. At 72 h p.i., cells were fixed and stained for lamin B (green) and Cdk-1 (red). The insets indicate magnified (×3) sections of the respective images. (C) Cells showing colocalization (shaded bars) or no colocalization (unshaded bars) for the cellular kinases and lamin B were counted among the infected (I) and mock-infected (M) samples. The data were analyzed using two-tailed Fisher's exact test, and the P values for the differences between the mock-infected and infected samples are shown.