FIG 8.

Association of UL97 with UL50 and UL53. (A) HFFs were infected with FLAG-UL97 BADGFP at an MOI of 1. At 72 h p.i., cells were fixed and stained for FLAG (green) and either UL50 (red) (i to iv) or UL53 (red) (v to viii). The samples were visualized using confocal microscopy. The insets indicate magnified (×3) sections of the respective images. Arrows show areas of colocalization. (B) Nuclear lysates were obtained at 72 h p.i. from HFF cells infected with HCMV AD169-RV (WT) or UL53-FLAG AD169-RV (53F) at an MOI of 1. Lysates were precleared and incubated with anti-FLAG M2 monoclonal antibody-conjugated agarose beads. Bound proteins were eluted using low pH and analyzed by Western blotting using antibodies against the proteins indicated to the right of the panel.