TABLE 1.
Summary of HCMV AD169 BAC constructs
| Construct | Genetic background | Reference for background construct | Change(s) introduced |
|---|---|---|---|
| 50N pBADGFP | pBADGFP | 37 | UL50 residues Ala45, Met46, and Leu47 were mutated to stop codons, and an additional nucleotide was added after the third stop codon to introduce a translational frameshift |
| 50NR pBADGFP | UL50 Null pBADGFP | This study | Restored the WT UL50 coding sequence to the UL50 null construct |
| 53N pBADGFP | pBADGFP | 37 | UL53 residues Met81, Met82, and Met84 were mutated to stop codons, and nucleotide 252 was deleted to introduce a translational frameshift |
| 53NR pBADGFP | UL53 Null pBADGFP | This study | Restored the WT UL53 coding sequence to the UL53 null construct |
| 53-F AD169-RV | AD169-RV | 42 | FLAG sequence at C terminus of UL53 |
| 53-F pBADGFP | pBADGFP | 37 | FLAG sequence at C terminus of UL53 |
| 50N 53-F pBADGFP | UL50 Null pBADGFP | This study | FLAG sequence at C terminus of UL53 |
| FLAG-97 pBADGFP | pBADGFP | 37 | FLAG sequence at N terminus of UL97 |
| 50N FLAG-97 pBADGFP | UL50 Null pBADGFP | This study | FLAG sequence at N terminus of UL97 |
| 53N FLAG-97 pBADGFP | UL53 Null pBADGFP | This study | FLAG sequence at N terminus of UL97 |
| 50NR FLAG-97 pBADGFP | UL50 Null Rescue pBADGFP | This study | FLAG sequence at N terminus of UL97 |
| 53NR FLAG-97 pBADGFP | UL53 Null Rescue pBADGFP | This study | FLAG sequence at N terminus of UL97 |