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. 2014 Jan;88(1):745–751. doi: 10.1128/JVI.02568-13

FIG 5.

FIG 5

UBE2M is essential for Vpx-mediated SAMHD1 degradation. (A) A UBE2M mutant inhibited Vpx-mediated SAMHD1 degradation. In HEK293T cells, SIVmac239 Vpx failed to induce SAMHD1 degradation in the presence of increasing amounts of UBE2M(C111S). Cell lysates were harvested and analyzed by SDS-PAGE, followed by immunoblotting to detect SAMHD1-HA. β-Actin was used as the loading control. (B) The UBE2F mutant did not inhibit Vpx-mediated SAMHD1 degradation. (C) Silencing of UBE2M inhibited the Vpx-mediated degradation of SAMHD1. (D) Schematic diagram of Nedd8-activated Vpx-CRL4 E3 ligase to induce SAMHD1 degradation.