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Recombinant UL28 virus constructs. The HSV-1 genome is shown at the top, with the long and short unique regions represented as UL and US, respectively. The UL28 open reading frame from wild-type KOS or each recombinant virus is expanded below, with virus names and protein sizes indicated on the left of each construct. The amino acid numbers below each construct indicate protein lengths. The vFH499 virus contains a nonsense mutation of amino acid 741.
