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Recombinant NTAP-UL28 virus single-step growth curves. Vero (A) or CV28 (B) cells were infected with the indicated HSV-1 recombinant viruses or with wild-type (KOS) virus at an MOI of 1 at 4°C for 1 h and incubated at 37°C. The cultures were harvested at the indicated times postinfection and freeze-thawed, and the virus yield at each time point was determined by the plaque titer on CV28 cells.
