FIG 4.

Administration of rapamycin has no effect on NDV infection in DF-1 cells. (A) DF-1 cells were pretreated with rapamycin (100 nm) or DMSO (control) in complete medium for 4 h and then processed as described for Fig. 3A. (B) At 18 and 24 hpi, extracellular virus yields were determined by TCID₅₀ in DF-1 cells and expressed as TCID₅₀/ml. (C) DF-1 cells were pretreated with rapamycin or DMSO (control) and then processed as described for panel A, except that the concentration of drug varied (50, 100, 500, and 1,000 μM; wedge). (D) At 18 hpi, extracellular virus yields were determined by TCID₅₀ in DF-1 cells and expressed as TCID₅₀/ml. Data are presented as means from three independent experiments. Significance is analyzed with two-tailed Student's t test (#, P > 0.05), compared to the control group.