FIG 2.

Time course of viral protein synthesis determined by pulse-labeling with radioactive amino acids. (A and B) BS-C-1 cells (A) and MEFs (B) were mock infected or infected with 5 PFU/cell of WT VACV or vD10rev, vΔD10, vD10mu, and vD10stop mutant viruses. At the indicated time after infection (shown in hours), the cells were pulse-labeled with [³⁵S]methionine and [³⁵S]cysteine for 30 min. The cells were harvested and lysed, and proteins were resolved by SDS-polyacrylamide gel electrophoresis and exposed to a PhosphorImager for visualization of newly synthesized proteins. The positions and masses (in kilodaltons) of marker proteins are indicated to the left of the gels.