FIG 3.

Time course of synthesis of specific early, intermediate, and late viral proteins. (A and B) BS-C-1 cells (A) and MEFs (B) were infected with 5 PFU/cell of WT VACV or vD10rev, vΔD10, vD10mu, or vD10stop and harvested at the indicated time (shown in hours). The lysates were analyzed by Western blotting using antibodies to the E3 early protein, the D13 intermediate protein, and the A3 late protein. Antibody to actin served as a loading control. One set of cells was infected in the presence of 1-β-d-arabinofuranosylcytosine (AraC) to inhibit viral DNA replication and confirm the stage of expression of the viral proteins.