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. 2014 Jan;88(1):176–187. doi: 10.1128/JVI.02098-13

FIG 2.

FIG 2

Functional avidity was determined in an IFN-γ ELISpot assay using peptide dilutions and defined as the peptide concentration required to induce 50% of the maximal response (EC50%). (A) Functional avidity was measured for HLA-B*57-restricted peptides (black circles for peptides derived from Gag protein and gray triangles for peptides derived from Nef protein), HLA-B57-nonrestricted peptides in HLA-B*57+ subjects (gray circles), and HLA-B*57-nonrestricted peptides in HLA-B*57− subjects (clear circles). (B) Functional avidity is correlated with the frequency of HIV-specific CD8+ T cells measured by ELISpot IFN-γ assay for HLA-B*57-restricted peptides (left panel) but not for HLA-B*57-nonrestricted peptides (right panel) whether in HLA-B*57+ (gray circles) or HLA-B*57− subjects (clear circles).