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TEM of filovirus VLPs. Purified VLPs produced from 293T cells transfected with plasmids expressing LLOV (A, B, E, and F), EBOV (Zaire) (C and G), and MARV (Angola) (D and H) proteins were fixed and stained as described in Materials and Methods. For immuno-TEM (B and F), an anti-LLOV GP monoclonal antibody was used. Scale bars represent 500 nm (A to D) and 200 nm (E to H). Arrowheads indicate gold particles.
