FIG 5.

Effects of chemical inhibitors on infectivities of pseudotyped VSVs. Vero E6 cells were pretreated with ammonium chloride (AMC) (A), monensin (MON) (B), and cathepsin B (CatB) (C) and L (CatL) (D) inhibitors for 30 min at 37°C. The treated cells were then infected with VSVΔG*-Zaire (Zaire), VSVΔG*-Angola (Angola), VSVΔG*-Lloviu (Lloviu), and VSVΔG*-G (VSV) appropriately diluted to yield 200 to 2,000 IUs/10⁶ cells. At 20 h postinoculation, GFP-positive cells were counted using an IN Cell Analyzer 2000 (GE Healthcare). The percentages of infectivity were determined by setting the number of the untreated cells to 100%. Each experiment was performed three times, and averages and standard deviations are shown.