FIG 5.

Pefabloc SC inhibits SGE-mediated enhancement of dengue and West Nile viruses in vivo. Four groups of 10 IFNAR^−/− C57BL/6 mice were inoculated into a single rear footpad with 20 μl containing 10⁷ genome equivalents (GE) of DENV2 and 1% DMSO (D), 10⁷ GE DENV2 with 1 mM Pefabloc SC in DMSO (D+P), 10⁷ GE DENV2 with 1.0 SGE and 1% DMSO (D+S), or 10⁷ GE with 1 mM Pefabloc SC in DMSO and 1.0 SGE (D+P+S). Twenty-four hpi, footpads (A), lymph nodes (B), blood (C), and spleens (D) were harvested into RLT buffer, and RNA was extracted for RT-qPCR analysis. DENV vRNA was normalized to total RNA per reaction. (E) C57BL/6 mice were inoculated in a single rear footpad (7 per group) with 500 PFU WNV and 1% DMSO (W) or with 1 mM Pefabloc SC in DMSO (W+P). Relative viral load was assessed in blood by RT-qPCR 24 hpi. (F) Three groups of 10 C57BL/6 mice were inoculated in rear footpads with 500 PFU WNV and 1% DMSO (W), with 1.0 C. tarsalis SGE and 1% DMSO (W+S), or with both 1.0 C. tarsalis SGE and 1 mM Pefabloc SC in DMSO (W+P+S). Relative viral load was assessed in footpads by RT-qPCR 24 hpi. Each symbol represents one mouse. The dotted line represents the background threshold. Mann-Whitney analysis was performed to assess statistical significance between groups. Data represent one experiment.