FIG 6.

RT-qPCR analysis of TH2/TH1 cytokine expression in footpads and lymph nodes from IFNAR^−/− C57BL/6 mice infected with DENV2 and treated with SGE and Pefabloc SC. Relative expression of IL-4 (A and B), IL-5 (C and D), IL-6 (E and F), IL-10 (G and H), IFN-γ (I and J), and TNF-α (K and L) was examined in footpads and draining lymph nodes 24 hpi in mice inoculated into a single rear footpad with 20 μl containing 10⁷ genome equivalents (GE) of either DENV2 and 1% DMSO (D), 10⁷ GE DENV2 with 1 mM Pefabloc SC in DMSO (D+P), 10⁷ GE DENV2 with 1.0 SGE (D+S), or 10⁷ GE with 1 mM Pefabloc SC in DMSO and 1.0 SGE (D+S+P). Relative cytokine levels were normalized to GAPDH. Each dot represents a single mouse. Mann-Whitney tests were performed between groups to assess statistical significance. Data represent one experiment.