TABLE 2.
Relative RT-qPCR data from SGE treatments of mouse embryonic fibroblasts followed by infection with multiple dengue virus serotypes and isolatesa
| Isolate | Result by SGE level |
P valuee | |
|---|---|---|---|
| 0 | 1.0 | ||
| DENV1 Hawaiib (m) | 1.1 (± 0.0) | 4.5 (±0.3) | <0.05 |
| DENV2 TH-36 (m) | 0.9 (± 0.0) | 10.6 (±1.6) | <0.05 |
| DENV3 CAESc (tc) | 1.7 (± 0.2) | 14.2 (±0.4) | <0.05 |
| DENV4 H241 (tc) | 1.0 (± 0.0) | 51.3 (±3.5) | <0.05 |
| DENV2 NGC (tc) | 1.0 (± 0.1) | 12.9 (±0.3 | <0.05 |
| DENV2 PLO46d (tc) | 1.2 (± 0.3) | 30.0 (±3.9) | <0.05 |
| DENV2 E124/E128 (tc) | 1.7 (± 0.4) | 1.9 (±0.3) | NS |
Data represent averages from at least three independent in vitro SGE-mediated enhancement assays (see Materials and Methods). Untreated samples were set to 1.0. Student's t tests were performed to assess statistical significance.
Mouse-adapted DENV types 1 and 2 were obtained from the ATCC as detailed in Materials and Methods.
Tissue culture-adapted DENV types 2 to 4 were obtained from the ATCC and the Connecticut Agricultural Experiment Station as detailed in Materials and Methods.
Stocks from infectious clones of tissue culture-adapted DENV type 2 PLO46 and E124/128 were obtained from Sujan Shresta.
Data represent averages from three or more independent experiments and include standard errors of the means. Student's t tests were performed to assess statistical significance. NS, not significant.