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Detection of dual marker expression in infected cell culture. (A) Reconstitution of vBRFseR on Vero cells using FWPV as a helper virus. Expression of both mRFP and eGFP can be detected upon reconstitution. (B) Expression of mRFP and eGFP during infection. mRFP can be detected at 6 h p.i.; eGFP can be detected at 12 h p.i.; araC can block eGFP but not mRFP expression in infected cells, as evidenced by the expression of mRFP only. Scale bars, 200 μm.
