TABLE 1.

CPXV-BR ORFs, mutant BACs, and reconstitution

CPXV-BR gene	VACV-COP homolog(s)	Function of VACV homologsa	Mutant BAC	Reconstitution	Conservationb
CPXV001		Unknown
CPXV002		Unknown
CPXV003	C23L	Chemokine binding protein
CPXV004		Unknown
CPXV005	C22L	Tumor necrosis factor receptor
CPXV006	C19L	Ankyrin
CPXV007		Unknown
CPXV008	C17L	Ankyrin
CPXV009	C16L	Unknown
CPXV010	N2L	Alpha-amanitin sensitivity protein	pBRFseR d10	Yes	No
CPXV011		Ankyrin
CPXV012		Unknown	pBRFseR d12	Yes	No
CPXV013		Kelch like	pBRFseR d13	Yes	No
CPXV014f	C22L, B28R	Tumor necrosis factor receptor	pBRFseR d14	Yes	No
CPXV015f		Unknown	pBRFseR d15	Yes	No
CPXV016		Ankyrin
CPXV017		Ankyrin
CPXV018		Unknown	pBRFseR d18	Yes	No
CPXV019		Ankyrin
CPXV020		Unknown	pBRFseR d20	Yes	No
CPXV021	C11R	Epidermal growth factor	pBRFseR d21	Yes	No
CPXV022	C10L, C4L	Interleukin-1 receptor antagonist	pBRFseR d22	Yes	No
CPXV023		Ubiquitin ligase/host defense modulator protein	pBRFseR d23	Yes	No
CPXV024		Interleukin-18 binding protein	pBRFseR d24	Yes	No
CPXV025		Ankyrin host range protein
CPXV026		Unknown	pBRFseR d26	Yes	No
CPXV027	C9L	Ankyrin
CPXV028	C8L	Unknown	pBRFseR d28	Yes	No
CPXV029	C7L	Host range virulence factor	pBRFseR d29	Yes	No
CPXV030	C6L	Unknown	pBRFseR d30	Yes	No
CPXV031f	C5L	Unknown	pBRFseR d31	Yes	No
CPXV032	C5L	Unknown	pBRFseR d32	Yes	No
CPXV033	C10L, C4L	Interleukin-1 receptor antagonist	pBRFseR d33	Yes	No
CPXV034	C3L	Complement binding protein (secreted)	pBRFseR d34	Yes	No
CPXV035	C2L	Kelch-like protein	pBRFseR d35	Yes	No
CPXV036	C1L	Unknown	pBRFseR d36	Yes	No
CPXV037	N1L	Virokine/NF-κB inhibitor	pBRFseR d37	Yes	No
CPXV038	N2L	Alpha amanitin sensitivity protein	pBRFseR d38	Yes	No
CPXV039	M1L	Ankyrin
CPXV040	M2L	NF-κB inhibitor	pBRFseR d40	Yes	No
CPXV041	K1L	Ankyrin and NF-κB inhibitor
CPXV042	C12L, K2L, B13R, B14R	“Serpin 1,2,3”	pBRFseR d42	Yes	No
CPXV043	K3L	Interferon resistance and eIF2 alpha-like PKR inhibitor	pBRFseR d43	Yes	No
CPXV044	K4L	Nicking/joining enzyme	pBRFseR d44	Yes	No
CPXV045	K5L, K6L	Monoglyceride lipase (putative)	pBRFseR d45	Yes	No
CPXV046	K7R	Unknown	pBRFseR d46+47	Yes	No
CPXV047	Pseudo?c	Unknown
CPXV048	F1L	Apoptosis inhibitor (associated with mitochondria)	pBRFseR d48	Yes	No
CPXV049	F2L	dUTPase	pBRFseR d49	Yes	No
CPXV050	F3L	Kelch-like protein	pBRFseR d50	Yes	No
CPXV051	F4L	Ribonucleotide reductase small subunit	pBRFseR d51	Yes	No
CPXV051A	Pseudo?	Unknown
CPXV052	F5L	Membrane protein (36 kDa)	pBRFseR d52	Yes	No
CPXV053	F6L	Unknown	pBRFseR d53	Yes	No
CPXV054	F7L	Unknown	pBRFseR d54	Yes	No
CPXV055	F8L	Cytoplasmic protein	pBRFseR d55	Yes	No
CPXV056	F9L	Disulfide bond formation pathway protein	pBRFseR d56	No	P
CPXV057	F10L	Serine/threonine kinase	pBRFseR d57+58	No	P
CPXV058	F_ORF_D Pseudo?	Unknown
CPXV059	F11L	Unknown	pBRFseR d59	Yes	No
CPXV060	F12L	IEV-associated protein	pBRFseR d60	Yes	C
CPXV061	F13L	EEV phospholipase	pBRFseR d61	Yes	C
CPXV062	F14L	Unknown	pBRFseR d62	Yes	No
CPXV063		Unknown	pBRFseR d63	Yes	No
CPXV064	F15L	Unknown	pBRFseR d64	Yes	C
CPXV065	F16L	Unknown	pBRFseR d65	Yes	No
CPXV066	F17R	DNA binding phosphoprotein	pBRFseR d66	No	C
CPXV067	E1L	Poly(A) polymerase large subunit	pBRFseR d67	No	P
CPXV068	E2L	Unknown	pBRFseR d68	Yes	C
CPXV069	E3L	Interferon resistance and PKR inhibitor	pBRFseR d69	Yes	No
CPXV070	E4L	RNA polymerase 30 subunit	pBRFseR d70	No	C
CPXV071	E5R	Virosome component protein	pBRFseR d71	Yes	No
CPXV072	E6R	required for the formation of immature virions	pBRFseR d72	No	P
CPXV073	E7R	EEV myristylated soluble protein	pBRFseR d73	Yes	No
CPXV074	E8R	Endoplasmic reticulum localized membrane protein	pBRFseR d74	Yes	C
CPXV075	E9L	DNA polymerase	pBRFseR d75	No	P
CPXV076	E10R	Disulfide bond formation pathway protein	pBRFseR d76	No	P
CPXV077	E11L	Virion core protein	pBRFseR d77	No	No
CPXV078	O1L	Unknown	pBRFseR d78	Yes	No
CPXV078A	Pseudo?	Unknown
CPXV079	O2L	Glutaredoxin 1	pBRFseR d79	Yes	No
CPXV080	I1L	DNA binding protein	pBRFseR d80	No	C
CPXV081	I2L	Unknown	pBRFseR d81	No	C
CPXV082	I3L	DNA binding phosphoprotein	pBRFseR d82	No	C
CPXV083	I4L	Ribonucleotide reductase large subunit	pBRFseR d83	Yes	No
CPXV084	I5L	IMV protein	pBRFseR d84	Yes	C
CPXV085	I6L	Telomere binding protein	pBRFseR d85	Yes	C
CPXV086	I7L	Virion core protease	pBRFseR d86	No	P
CPXV087	I8R	RNA helicase/NPH-II	pBRFseR d87	No	P
CPXV088	G1L	Metalloprotease (predicted)	pBRFseR d88	No	P
CPXV089	G3L	virus fusion complex	pBRFseR d89	No	C
CPXV090	G2R	Viral late transcription factor	pBRFseR d90	No	C
CPXV091	G4L	Glutaredoxin 2	pBRFseR d91	No	C
CPXV092	G5R	Unknown	pBRFseR d92	No	P
CPXV093	G5.5R	RNA polymerase 7 subunit	pBRFseR d93	No	C
CPXV094	G6R	Unknown	pBRFseR d94	Yes	P
CPXV095	G7L	Virion assembly protein	pBRFseR d95	No	C
CPXV096	Pseudo?	Unknown
CPXV097	G8R	Viral late transcription factor 1	pBRFseR d96+97	No	C
CPXV098	G9R	Entry fusion complex protein	pBRFseR d98	No	P
CPXV099	L1R	IMV myristylated membrane protein	pBRFseR d99	No	P
CPXV100	L2R	Crescent formation	pBRFseR d100	No	C
CPXV101	L3L	Internal virion protein	pBRFseR d101	No	P
CPXV102	L4R	Core package and transcription protein	pBRFseR d102	No	P
CPXV103	L5R	IMV entry and fusion protein	pBRFseR d103	No	P
CPXV104	J1R	Virion morphogenesis protein	pBRFseR d104	No	C
CPXV105	J2R	Thymidine kinase
CPXV106	J3R	Poly(A) polymerase small subunit	pBRFseR d106	No	P
CPXV107	J4R	RNA polymerase 22 subunit	pBRFseR d107	No	C
CPXV108	J5L	Unknown membrane protein	pBRFseR d108	Yes	P
CPXV109	J6R	RNA polymerase 147 subunit	pBRFseR d109	No	P
CPXV110	H1L	Serine/Tyrosine phosphatase	pBRFseR d110	Yes	C
CPXV111	H2R	Entry and cell to cell fusion protein	pBRFseR d111	No	P
CPXV112	H3L	IMV heparin binding surface protein	pBRFseR d112	Yes	P
CPXV113	H4L	RNA polymerase associated protein RAP94	pBRFseR d113	No	P
CPXV114	H5R	Viral late transcription factor 4	pBRFseR d114	No	C
CPXV115	H6R	Topoisomerase type I	pBRFseR d115	Yes	P
CPXV116	Pseudo?	Unknown
CPXV117	H7R	Crescent formation	pBRFseR d117+116	No	C
CPXV118	D1R	Large capping enzyme	pBRFseR d118	No	P
CPXV119	D2L	Virion core	pBRFseR d119+119A	No	C
CPXV119A	D_ORF_B	Unknown
CPXV120	D3R	Virion core protein	pBRFseR d120	No	C
CPXV121	D4R	Uracil DNA glycosylase	pBRFseR d121	No	P
CPXV122	D5R	NTPase and DNA replication protein	pBRFseR d122	No	P
CPXV123	D6R	Viral early transcription factor small subunit	pBRFseR d123	No	P
CPXV124	D7R	RNA polymerase 18 subunit	pBRFseR d124	No	P
CPXV125	D8L	Carbonic anhydrase	pBRFseR d125	Yes	No
CPXV126	D9R	NTP-PPH containing mutT motif	pBRFseR d126	Yes	C
CPXV127	D10R	NPH-PPH RNA levels regulator containing mutT motif	pBRFseR d127	Yes	P
CPXV128	D11L	Helicase NPH-I	pBRFseR d128	No	P
CPXV129	D12L	Small capping enzyme	pBRFseR d129+130	No	P
CPXV130	Pseudo?	Unknown
CPXV131	D13L	Virion coat protein rifampin resistance	pBRFseR d131	No	P
CPXV132	A1L	Viral late transcription factor 2	pBRFseR d132	No	P
CPXV133	A2L	Viral late transcription factor 3	pBRFseR d133	No	P
CPXV134	A2.5L	Thioredoxin like protein	pBRFseR d134	No	C
CPXV135	A3L	P4b precursor	pBRFseR d135	No	P
CPXV136	A4L	Core protein	pBRFseR d136	Yes	C
CPXV137	A5R	RNA polymerase 19 subunit	pBRFseR d137	No	P
CPXV138	A6L	Virion morphogenesis protein	pBRFseR d138	No	C
CPXV139	A7L	Viral early transcription factor large subunit	pBRFseR d139	No	P
CPXV140	A8R	Viral intermediate transcription factor 3	pBRFseR d140	No	C
CPXV141	A9L	Membrane protein	pBRFseR d141	No	P
CPXV142	A10L	P4a precursor	pBRFseR d142	No	P
CPXV143	A11R	Membrane formation protein	pBRFseR d143	No	P
CPXV144	A12L	Structural protein	pBRFseR d144	No	C
CPXV145	A13L	Virion maturation protein	pBRFseR d145	No	C
CPXV146	A14L	IMV membrane protein (phosphorylated)	pBRFseR d146	No	C
CPXV147	A14.5L	IMV virulence factor (membrane protein)	pBRFseR d147	Yes	C
CPXV148	A15L	Unknown	pBRFseR d148	No	C
CPXV149	A16L	Entry and cell-to-cell fusion protein (myristilated)	pBRFseR d149	No	P
CPXV150	A17L	IMV membrane protein phosphorylated	pBRFseR d150	No	C
CPXV151	A18R	DNA helicase	pBRFseR d151	No	P
CPXV152	A19L	Unknown	pBRFseR d152+152A	No	C
CPXV152A	Pseudo?	Unknown
CPXV153	A21L	Entry and cell-to-cell fusion protein	pBRFseR d153	No	P
CPXV154	A20R	DNA processivity factor	pBRFseR d154	No	C
CPXV155	A22R	Holliday junction resolvase	pBRFseR d155	Yes	P
CPXV156	A23R	Viral intermediate transcription factor 3 (45-kDa subunit)	pBRFseR d156	No	P
CPXV157	A24R	RNA polymerase 132 subunit	pBRFseR d157	No	P
CPXV158	A25L	A-type inclusion protein	pBRFseR d158	Yes	No
CPXV159	A26L	P4c precursor	pBRFseR d159	Yes	No
CPXV160	Pseudo?	Unknown
CPXV161	A26L	P4c precursor	pBRFseR d161	Yes	No
CPXV162	A27L	Fusion protein	pBRFseR d162	Yes	No
CPXV163	A28L	IMV virus entry (membrane protein)	pBRFseR d163	No	P
CPXV164	A29L	RNA polymerase 35 subunit	pBRFseR d164	No	P
CPXV165	A30L	Virion morphogenesis protein	pBRFseR d165	No	C
CPXV166	A31R	Unknown	pBRFseR d166	Yes	No
CPXV167	A32L	DNA packaging and ATPase protein	pBRFseR d167	No	P
CPXV168	A33R	EEV glycoprotein	pBRFseR d168	Yes	No
CPXV169	A34R	EEV C-type lectin-like protein	pBRFseR d169	Yes	C
CPXV170	Pseudo?	Unknown	pBRFseR d170+171	Yes	No
CPXV171	A35R	Unknown
CPXV172	A36R	IEV specific	pBRFseR d172	Yes	No
CPXV173	A37R	Unknown	pBRFseR d173	Yes	No
CPXV174		Unknown	pBRFseR d174	Yes	No
CPXV175	A38L	CD47-like protein	pBRFseR d175	Yes	No
CPXV176	A39R	Semaphorin	pBRFseR d176	Yes	No
CPXV177	A40R	Lectin homolog	pBRFseR d177	Yes	No
CPXV178	A41L	Secreted virulence factor	pBRFseR d178	Yes	No
CPXV179	A42R	Profilin homolog	pBRFseR d179	Yes	No
CPXV180	A43R	Membrane glycoprotein class I	pBRFseR d180	Yes	No
CPXV181		Unknown	pBRFseR d181	Yes	No
CPXV182	A44L	Hydroxysteroid dehydrogenase	pBRFseR d182	Yes	No
CPXV183	A45R	Superoxide dismutase-like protein	pBRFseR d183	Yes	No
CPXV184	A46R	Interleukin-1 signaling inhibitor	pBRFseR d184	Yes	No
CPXV185	A47L	Unknown	pBRFseR d185	Yes	No
CPXV186	A48R	Thymidylate kinase	pBRFseR d186	Yes	No
CPXV187	A49R	Phosphotransferase anion transport protein (putative)	pBRFseR d187	Yes	No
CPXV188	A50R	DNA ligase	pBRFseR d188	Yes	No
CPXV189	A51R	Unknown	pBRFseR d189	Yes	No
CPXV190	A52R	Intracellular TLR and interleukin-1 signaling inhibitor	pBRFseR d190	Yes	No
CPXV191	A53R, A_ORF_T	Tumor necrosis factor receptor (CrmC)	pBRFseR d191	Yes	No
CPXV192	Pseudo?	Unknown
CPXV193	A55R	Kelch-like protein	pBRFseR d193	Yes	No
CPXV194	A56R	Hemagglutinin	pBRFseR d194	Yes	No
CPXV195	A57R	Guanylate kinase	pBRFseR d195	No	No
CPXV196	B1R	Serine/threonine kinase	pBRFseR d196	Yes	No
CPXV197	B2R; B3R	Schlafen	pBRFseR d197	Yes	No
CPXV198	B4R	Ankyrin
CPXV199	B5R	EEV complement control protein	pBRFseR d199	Yes	No
CPXV200	B6R	Unknown	pBRFseR d200	Yes	No
CPXV201	B7R	Virulence factor (endoplasmic reticulum associated)	pBRFseR d201	Yes	No
CPXV202	B8R	Interferon gamma receptor	pBRFseR d202	Yes	No
CPXV203	B9R	Virulence factor	pBRFseR d203	Yes	No
CPXV204	B10R	Kelch-like protein	pBRFseR d204	Yes	No
CPXV205	B11R	Unknown	pBRFseR d205	Yes	No
CPXV206	B12R	Serine/threonine kinase	pBRFseR d206	Yes	No
CPXV207	C12L, K2L, B13R, B14R	“Serpin 1,2,3”
CPXV208	C16L, B15R, B22R	Unknown	pBRFseR d208	Yes	No
CPXV209	B16R	Interleukin-1β receptor	pBRFseR d209	Yes	No
CPXV210	B17L	Unknown	pBRFseR d210	Yes	No
CPXV211	B18R	Ankyrin
CPXV212	B19R	Alpha/beta interferon receptor	pBRFseR d212	Yes	No
CPXV213	B20R	Ankyrin
CPXV214	Pseudo?	Unknown
CPXV215		Kelch-like protein	pBRFseR d214+215	Yes	No
CPXV216	C12L, K2L, B13R, B14R	Unknown	pBRFseR d216 (ATG)	Yes	No
CPXV217	C12L, K2L, B13R, B14R	“Serpin 1,2,3”	pBRFseR d217	Yes	No
CPXV218	C14L, C13L	Unknown	pBRFseR d218	Yes	No
CPXV219		Surface glycoprotein	pBRFseR d219	Yes	No
CPXV220	C21L, C20L, C19L, B25R, B26R, B27R	Ankyrin
CPXV221	CrmD	Tumor necrosis factor receptor (CrmD)	pBRFseR d221	Yes	No

^aInformation was obtained from the PBRC.

^bC, gene families conserved in chordopoxviruses; P, gene families conserved in poxviruses (32).

^cPseudo?, pseudogene (according to PBRC).