. 2014 Jan;88(1):21–40. doi: 10.1128/JVI.01558-13

TABLE 4.

Infectivity and sensitivity to cold and sCD4 inactivation of viruses pseudotyped with d266 and d434 Env variants from monkey 354-05

Mutant	Residue at position:		Infectivity (%)^a	Cold IT₅₀ (h)^b	sCD4 reactivity^c
Mutant	317	440	Infectivity (%)^a	Cold IT₅₀ (h)^b	sCD4 reactivity^c
d266A	L	T	100	15	0.89
d266A L317W	W	T	102	43	0.15
d266A T440A	L	A	63	26	0.16
d266A L317W/T440A	W	A	255	50	0.11
d266B	W	T	100	76	0.46
d266B W317L	L	T	52	35	0.26
d266B T440A	W	A	52	84	0.14
d266B W317L/T440A	L	A	72	54	0.21
d434A	W	A	100	>96	0.09
d434A W317L	L	A	72	54	0.21
d434A A440T	W	T	86	77	0.14
d434A W317L/A440T	L	T	72	28	0.36
d434B	W	A	100	79	0.37
d434B W317L	L	A	61	47	0.26
d434B A440T	W	T	90	39	0.27
d434B W317L/A440T	L	T	41	23	0.84

Infectivity levels represent the percentage of mutant reporter virus readout at maximum virus input compared with the infectivity levels of the reporter viruses pseudotyped with the corresponding wild-type Env.

Cold IT₅₀ represents the half-life of virus on ice, in hours.

sCD4 reactivity (ER_sCD4) was calculated as described in Materials and Methods and is reported relative to the ER_sCD4 of KY Env, which is set at a value of 1.