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Incorporation of vdUTPase proteins into purifying virions. Immunoblots of electrophoretically separated virions of wild-type HSV-1(F) (lane 1), YK750 (ΔvdUTPase) (lane 2), YK751 (vdUTPaseS187A) (lane 3), or YK752 (vdUTPaseΔ/SA-repair) (lane 4) purified by sucrose gradient centrifugation and blotted with either anti-vdUTPase mouse polyclonal antibody or anti-VP5 mouse monoclonal antibody. A molecular mass marker (in kilodaltons) is shown on the left.
