FIG 7.

Effect of phosphorylation of vdUTPase Ser-187 on HSV-1 replication in SK-N-SH and HEp-2 cells. (A and B) HEp-2 (A) or SK-N-SH (B) cells were infected with wild-type HSV-1(F), YK750 (ΔvdUTPase), YK751 (vdUTPaseS187A), YK752 (vdUTPaseΔ/SA-repair), YK759 (vdUTPaseD97A), YK760 (vdUTPaseD97A-repair), YK753 (vdUTPaseS187D), or YK754 (vdUTPaseS187D-repair) at an MOI of 5. Total virus from the cell culture supernatants and infected cells was harvested at 36 h postinfection, and virus titers were assayed. Each value is the mean ± standard error for six experiments. (C) SK-N-SH cells were infected with wild-type HSV-1(F), YK511 (Us3K220M), or YK513 (Us3K220M-repair) at an MOI of 5. The experiments were performed as described for panel B. Each value is the mean ± standard error for three experiments. “P” represents the statistical significance value according to the two-tailed Student's t test. Data are representative of three independent experiments.