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. 1971 Jan;68(1):116–120. doi: 10.1073/pnas.68.1.116

Mitotic Separation of Two Human X-Linked Genes in Man—Mouse Somatic Cell Hybrids

O J Miller *, P R Cook ‡, P Meera Khan §, S Shin §, M Siniscalco §
PMCID: PMC391174  PMID: 5276281

Abstract

Six interspecific somatic hybrid cell lines were derived from a mouse line deficient in hypoxanthine: guanine phosphoribosyltransferase (HGPRT) and human diploid cells with normal enzyme activity. Human HGPRT was present in all six hybrids and the clones derived from them. However, in two of the six, and in some clones from another two, human glucose-6-phosphate dehydrogenase (G6PD) was absent. Since the structural loci for both these enzymes are X-linked in man, these findings suggest that these two loci have separated quite frequently through chromosome breakage and that they must be rather far apart on the X chromosome.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DAVIDSON R. G., NITOWSKY H. M., CHILDS B. DEMONSTRATION OF TWO POPULATIONS OF CELLS IN THE HUMAN FEMALE HETEROZYGOUS FOR GLUCOSE-6-PHOSPHATE DEHYDROGENASE VARIANTS. Proc Natl Acad Sci U S A. 1963 Sep;50:481–485. doi: 10.1073/pnas.50.3.481. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. HARRIS H., WATKINS J. F. HYBRID CELLS DERIVED FROM MOUSE AND MAN: ARTIFICIAL HETEROKARYONS OF MAMMALIAN CELLS FROM DIFFERENT SPECIES. Nature. 1965 Feb 13;205:640–646. doi: 10.1038/205640a0. [DOI] [PubMed] [Google Scholar]
  3. Harris H., Cook P. R. Synthesis of an enzyme determined by an erythrocyte nucleus in a hybrid cell. J Cell Sci. 1969 Jul;5(1):121–133. doi: 10.1242/jcs.5.1.121. [DOI] [PubMed] [Google Scholar]
  4. LITTLEFIELD J. W. THREE DEGREES OF GUANYLIC ACID--INOSINIC ACID PYROPHOSPHORYLASE DEFICIENCY IN MOUSE FIBROBLASTS. Nature. 1964 Sep 12;203:1142–1144. doi: 10.1038/2031142a0. [DOI] [PubMed] [Google Scholar]
  5. Littlefield J. W. The use of drug-resistant markers to study the hybridization of mouse fibroblasts. Exp Cell Res. 1966 Jan;41(1):190–196. doi: 10.1016/0014-4827(66)90558-1. [DOI] [PubMed] [Google Scholar]
  6. MARKS P. A., GROSS R. T. Erythrocyte glucose-6-phosphate dehydrogenase deficiency: evidence of differences between Negroes and Caucasians with respect to this genetically determined trait. J Clin Invest. 1959 Dec;38:2253–2262. doi: 10.1172/JCI104006. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Matsuya Y., Green H., Basilico C. Properties and uses of human-mouse hybrid cell lines. Nature. 1968 Dec 21;220(5173):1199–1202. doi: 10.1038/2201199a0. [DOI] [PubMed] [Google Scholar]
  8. Migeon B. R., Der Kaloustian V. M., Nyhan W. L., Yough W. J., Childs B. X-linked hypoxanthine-guanine phosphoribosyl transferase deficiency: heterozygote has two clonal populations. Science. 1968 Apr 26;160(3826):425–427. doi: 10.1126/science.160.3826.425. [DOI] [PubMed] [Google Scholar]
  9. Migeon B. R., Miller C. S. Human-mouse somatic cell hybrids with single human chromosome (group E): link with thymidine kinase activity. Science. 1968 Nov 29;162(3857):1005–1006. doi: 10.1126/science.162.3857.1005. [DOI] [PubMed] [Google Scholar]
  10. Migeon B. R., Smith S. W., Leddy C. L. The nature of thymidine kinase in the human-mouse hybrid cell. Biochem Genet. 1969 Dec;3(6):583–590. doi: 10.1007/BF00485479. [DOI] [PubMed] [Google Scholar]
  11. Motulsky A. G., Vandepitte J., Fraser G. R. Population genetic studies in the Congo. I. Glucose-6-phosphate dehydrogenase deficiency, hemoglobin S, and malaria. Am J Hum Genet. 1966 Nov;18(6):514–537. [PMC free article] [PubMed] [Google Scholar]
  12. Nabholz M., Miggiano V., Bodmer W. Genetic analysis with human--mouse somatic cell hybrids. Nature. 1969 Jul 26;223(5204):358–363. doi: 10.1038/223358a0. [DOI] [PubMed] [Google Scholar]
  13. Nyhan W. L., Bakay B., Connor J. D., Marks J. F., Keele D. K. Hemizygous expression of glucose-6-phosphate dehydrogenase in erythrocytes of heterozygotes for the Lesch-Nyhan syndrome. Proc Natl Acad Sci U S A. 1970 Jan;65(1):214–218. doi: 10.1073/pnas.65.1.214. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. SZYBALSKA E. H., SZYBALSKI W. Genetics of human cess line. IV. DNA-mediated heritable transformation of a biochemical trait. Proc Natl Acad Sci U S A. 1962 Dec 15;48:2026–2034. doi: 10.1073/pnas.48.12.2026. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Saksela E., Aula P., Cantell K. Chromosomal damage of human cells induced by Sendai virus. Ann Med Exp Biol Fenn. 1965;43(3):132–136. [PubMed] [Google Scholar]
  16. Scaletta L. J., Rushforth N. B., Ephrussi B. Isolation and properties of hybrids between somatic mouse and Chinese hamster cells. Genetics. 1967 Sep;57(1):107–124. doi: 10.1093/genetics/57.1.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Seegmiller J. E., Rosenbloom F. M., Kelley W. N. Enzyme defect associated with a sex-linked human neurological disorder and excessive purine synthesis. Science. 1967 Mar 31;155(3770):1682–1684. doi: 10.1126/science.155.3770.1682. [DOI] [PubMed] [Google Scholar]
  18. Weiss M. C., Green H. Human-mouse hybrid cell lines containing partial complements of human chromosomes and functioning human genes. Proc Natl Acad Sci U S A. 1967 Sep;58(3):1104–1111. doi: 10.1073/pnas.58.3.1104. [DOI] [PMC free article] [PubMed] [Google Scholar]

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