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. 2014 Jan;88(1):188–201. doi: 10.1128/JVI.02245-13

FIG 2.

FIG 2

Expression and interaction of viral proteins during ORF49 mutant virus infection. (A) Proteins expressed in mock-infected MeWo cells (lane 1), mock-infected MeWoORF49 cells (lane 2), rpOka-infected MeWo cells (lane 3), rpOkaORF49M1L-infected MeWo cells (lane 4), and rpOkaORF49M1LRev-infected MeWo cells (lane 5) were visualized with Abs against gH, ORF61p, α-tubulin, ORF44p, and ORF49p. (B) The interaction between ORF44p and ORF49p was analyzed in rpOkaORF49M1L-infected cells (lane 1) and rpOkaORF49M1LRev-infected cells (lane 2). Immunoprecipitates obtained with an anti-ORF49 Ab from each type of virus-infected cells were electrophoretically separated and visualized using anti-ORF44 and anti-ORF49 Abs. (C) The viral proteins incorporated into virions from rpOka-infected MeWo cells (lane 1), rpOkaORF49M1L-infected MeWo cells (lane 2), and rpOkaORF49M1L-infected MeWoORF49 cells (lane 3) were visualized using Abs against gH, ORF61p, ORF44p, and ORF49p. (D) Proteins expressed in rpOkaORF49-41AAAA44-infected MeWo cells (lane 1) and rpOkaORF49-41AAAA44Rev-infected MeWo cells (lane 2) were visualized using Abs against gH, ORF61p, α-tubulin, ORF44p, and ORF49p. (E) The interaction between ORF44p and ORF49p was analyzed in rpOkaORF49-41AAAA44-infected cells (lane 1) and rpOkaORF49-41AAAA44Rev-infected cells (lane 2). Immunoprecipitates obtained using an anti-ORF49 Ab from each type of virus-infected cells were electrophoretically separated and visualized with anti-ORF44 and anti-ORF49 Abs. (F) The viral proteins incorporated into virions from rpOkaORF49-41AAAA44-infected MeWo cells (lane 1), and rpOkaORF49-41AAAA44-infected MeWoORF49 cells (lane 2) were visualized with Abs against gH, ORF61p, ORF44p, and ORF49p.