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Localization and accumulation of ORF44 mutant proteins in MeWo cells expressing ORF49p. MeWo cells were cotransfected with CAG/ORF49 and CAG/ORF44T128A (A), CAG/ORF44F129A (B), CAG/ORF44K130A (C), CAG/FLAGORF44I121stop (D), CAG/FLAGORF44P136stop (E), or CAG/FLAGORF44F129AP136stop (F). Cells were fixed at 48 h posttransfection and triple labeled for ORF44 (green), ORF49 (red), and TGN46 (blue). Nuclei were stained with Hoechst 33342 (cyan). Scale bars, 5 μm.
