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Localization and accumulation of ORF44p in MeWo cells expressing ORF49 mutant proteins. MeWo cells were cotransfected with CAG/ORF44 and CAG/ORF49N48 (A), CAG/ORF49N44 (B), CAG/ORF49N40 (C), or CAG/ORF49-41AAAA44 (E), or transfected with CAG/ORF49-41AAAA44 alone (D). Cells were fixed at 48 h posttransfection and triple labeled for ORF44 (green), ORF49 (red), and TGN46 (blue). Nuclei were stained with Hoechst 33342 (cyan). Scale bars, 5 μm.
