TABLE 1.

Primers used for ORF44 or ORF49 mutations

Primer	Sequencea	Amino acid substitution(s)
ORF44 361at-ta	5′-TAT CCG GTT GAA AAC TAA GAC CAT GTT TTT GGA-3′	Ile 121 to stop (TAA)
ORF44 382a-g	5′-CAT GTT TTT GGA GCA GCG TTT AAG AAC CC-3′	Thr 128 to Ala
ORF44 385tt-gc	5′-TT TTT GGA GCA ACG GCT AAG AAC CCG ATC G-3′	Phe 129 to Ala
ORF44 388aa-gc	5′-TTT GGA GCA ACG TTT GCG AAC CCG ATC GCG-3′	Lys 130 to Ala
ORF44 406ccc-taa	5′-AAC CCG ATC GCG TAC TAA CTT CCA ACA TCT ATT-3′	Pro 136 to stop (TAA)
ORF49 M1L	5′-ATT GCG GTC ATT GCG TTG GGA CAA TCT TCA-3′	Met 1 to Leu
ORF49 41AAAA44	5′-TTT GAA GAC TTT GCC GCT GCT GCG AAT GTA ACA GAG-3′	Asp-Phe-Asp-Glu (41–44) to Ala-Ala-Ala-Ala

^aNucleotides that differ from those of the wild type are underlined.