FIG 6.

Competitive binding of vXCL1 and rat XCL1 to rat CD4⁻ DC. NycoPrep gradient-enriched rat DC were incubated with 400 ng/ml rat XCL1-APC in the presence of different concentrations of unlabeled vXCL1 (A) or with 400 ng/ml vXCL1-APC in the presence of different concentrations of unlabeled rat XCL1 (B), washed, and analyzed for binding of the respective fluorophore-tagged chemokine to rat DC (CD103⁺MHCII⁺). In both instances, binding of the labeled chemokine was effectively competed by the unlabeled chemokine on CD4⁻ DC (lower right quadrants). One representative experiment of two is shown.