FIG 5.

Interaction and colocalization of HA72 and P33. (A) Results from the yeast two-hybrid assay revealed an interaction of HA72 and P33. The AD fusion plasmid and the BD fusion plasmid were used to cotransform AH109 yeast competent cells. The names of the cotransforming plasmids are indicated. (B) Immunofluorescent staining or autofluorescence images, showing colocalization of HA72 and P33. HzAM1 cells were infected with vHaBacHZ8-p33-egfp-ph or vHaBacHZ8-p33HA-ph, and at 48 hpi HA72 was stained with rhodamine-conjugated antibody. Bars, 10 μm.