TABLE 1.
Primers used in the study
| Primers | Sequencea | Restriction site(s) |
|---|---|---|
| Oligo(dT)15 three-site adaptor primer | 5′-CTGATCTAGAGGTACCGGATCCTTTTTTTTTTTTTTT-3′ | |
| Three-site adaptor primer | 5′-CTGATCTAGAGGTACCGGATCC-3′ | |
| ha72-specific forward primer | 5′- ATGAATTTAGATATACCCTACGA-3′ | |
| ha72up-F | 5′-CCCGGTACCCGATAGAATTAAATTCTTTGTTCA-3′ | KpnI site |
| ha72up-R | 5′-CCCCTCGAGGTGGACTATATTCCGCTAAAAT-3′ | XhoI site |
| ha72down-F | 5′-GGGTCTAGACCGAGACGATCGTAGGGTA-3′ | XbaI site |
| ha72down-R | 5′-GGGTCTAGAGATATCTGTGTCCAGTCGCAAATACC-3′ | XbaI and EcoRI sites |
| ha72R-F | 5′-CGCCTCGAGAGCGACGTTCCTCAACGCT-3′ | XhoI site |
| ha72R-R | 5′-CGCGCTAGCGTCCGTGGTCAATTCGAAAAT-3′ | NheI site |
| ha72K22E-R | 5′-CTACGTTAAAATCGGATATAGCCAATTCTAGCGGAATATAG-3′ | |
| ha72K22E-F | 5′-CTATATTCCGCTAGAATTGGCTATATCCGATTTTAACGTAG-3′ | |
| ha72K22R-F | 5′-CTATATTCCGCTAAGATTGGCTATATCCGATTTTAACGTAG-3′ | |
| ha72K22R-R | 5′-CTACGTTAAAATCGGATATAGCCAATCTTAGCGGAATATAG-3′ | |
| ha39-F | 5′-GAAATGCGAATCAGACAGATTACTCG-3′ | |
| ha39-R | 5′-CGCAACCTAACATTTGAGAACACAC-3′ |
a
Sequences of restriction sites are underlined.