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. 2014 Jan;88(1):377–392. doi: 10.1128/JVI.02689-13

TABLE 2.

Primers used for cloning of expression plasmids and mutagenesis of the 3′UTR plasmids

Primer Sequence (5′–3′)a
ORF31-33-Mutant1-F CTCGAATTCAGAGACGTCAACCCTTTTGTTTGGCTC
ORF31-33-Mutant1-R GAGCCAAACAAAAGGGTTGACGTCTCTGAATTCGAG
ORF31-33-Mutant2-F CCACGCACGTCGAAGACGTCCTAACAGGAGTGCTC
ORF31-33-Mutant2-R GAGCACTCCTGTTAGGACGTCTTCGACGTGCGTGG
ORF71-73-Mutant1-F TATCTGATTTAATAAACTGATACAAGTTTTGTAAGAA
ORF71-73-Mutant1-R TTCTTACAAAACTTGTATCAGTTTATTAAATCAGATA
ORF71-73-Mutant2-F CCATCACACTCCCAACACTATCGCCATACACCATAGA
ORF71-73-Mutant2-R TCTATGGTGTATGGCGATAGTGTTGGGAGTGTGATGG
ORF71-73-Mutant3-F GCTGGGGGGCTCCCAAGTGGTGGACTTTTGGCACCAC
ORF71-73-Mutant3-R GTGGTGCCAAAAGTCCACCACTTGGGAGCCCCCCAGC
ORF71-73-Mutant4-F TGGCACCACGAGGTCAAGTGGCTGATTACAAAAGCC
ORF71-73-Mutant4-R GGCTTTTGTAATCAGCCACTTGACCTCGTGGTGCCA
3Flag-ORF30-F AAAGGGCCCTGGGTGAGCCAGTGGATCCTGGACA
3Flag-ORF31-F AAAGGGCCCTGTCACAAAACAGAAAGACTCTGCCT
3Flag-ORF32-F AATGGGCCCTGGATGCGCATGCTATCAACGAAAGA
3Flag-ORF33-F ATAGGGCCCTGGCTAGCCGGAGGCGCAAACTTC
3Flag-ORF30-33-Universal-R GCCAAGCTTTTAAGCAGTACTTCGGTTTATTTGA
3Flag-ORF73a-F TATGCTAGCGGCGCCCCCGGGAATGCGCCTGAGGT
3Flag-ORF73a-R AGTACGCTAGCCGTTGTCCGTGTGTTCA
3Flag-ORF73b-F TGAACACACGGACAACGGCTAGCGTACT
3Flag-ORF73b-F CCCCTCGAGTTCTTACAAAACTTGTTAGTGTTTATTAAATCAGA
3Flag-Luc-F GCGGCTAGCGGAAGACGCCAAAAACATAAAGAAAG
3Flag-Luc-F GCGGAATTCCGACTCTAGAATTACACGGCGATCT
ORF30-ApaI-F AAAGGGCCCTGGGTGAGCCAGTGGATCCTGGACA
ORF33-HindIII-R GCCAAGCTTTTAAGCAGTACTTCGGTTTATTTGA
ORF31-ApaI-F AAAGGGCCCTGTCACAAAACAGAAAGACTCTGCCT
ORF32-ApaI-F AATGGGCCCTGGATGCGCATGCTATCAACGAAAGA
ORF33-ApaI-F ATAGGGCCCTGGCTAGCCGGAGGCGCAAACTTC
a

Mutated nucleotides and restriction sites are indicated by underlining.

HHS Vulnerability Disclosure