FIG 7.

RNA-Seq analyses of RNA from C^KO mutant and parental virus-infected cells. cDNA libraries prepared using 10 μg total RNA from virus-infected cells depleted of ribosomal RNAs were analyzed by Ion Torrent sequencing as described in Materials and Methods. Diagrams show the alignment of reads to the viral genome sequence for parental MVvac-WT(GFP)-infected cells (top) and mutant MVvac-C^KO(GFP)-infected cells (bottom). Reads are separated corresponding to their polarity with +RNA shown in blue (mRNA, antigenome) and −RNA (genome) shown in red. Dashed lines correspond to gene borders.