TABLE 1.

MV-specific primers for qPCR analysis

Primer name	Sequence (5′→3′)	Binding sitea
Le-N fwd	CCAAACAAAGTTGGGTAAGG	2
Le-N rev	ACCGGATCCTGATGTAATGG	182r
N fwd	ATTGACACTGCAACGGAGTC	1530
N rev	GCCTTGTTCTTCCGAGATTC	1634r
N-P fwd	AACAACATCCGCCTACCATC	1707
N-P rev	TTCTGACCATGCTGCCATAG	1920r
P fwd	GTCGGGTTTGTTCCTGACAC	3154
P rev	AGGTAACGCTTCCGATCCTC	3248r
P-M fwd	GCCAGTCGACCCACCTAGTA	3360
P-M rev	CTTCCTGTCGCCTAGACCAG	3575r
M fwd	AACGCAAACCAAGTGT	3843
M rev	TGAAGGCCACTGCATT	4002r
M-F fwd	CCCCCTCTTCCTCAACACAAG	4755
M-F rev	TGGGTTCTGTTGGGTGTGTATG	4908r
F fwd	TGATTGCAGTGTGTCTTGGAG	6953
F rev	CCCGTAAGATCAGGCTTTAGG	7079r
F-H fwd	CTTCGTCATCAAGCAACCAC	7153
F-H rev	GCATTTATCCGGTCTCGTTG	7299r
H fwd	AACTCTGGTGCCGTCACTTC	8997
H rev	CCATCCCAGAGTGAGTGATATG	9068r
H-GFP fwd	CCCACTAGCCTACCCTCCAT	(9172)
H-GFP rev	GAACTTCAGGGTCAGCTTGC	(9401r)
GFP fwd	AGAACGGCATCAAGGTGAAC	(9736)
GFP rev	TGCTCAGGTAGTGGTTGTCG	(9870r)
GFP-L fwd	ACATGGTCCTGCTGGAGTTC	(9913)
GFP-L rev	AGTCCATAACGGGGAACCAC	9240r (10074r)
L fwd	AATCTCAAGTCCGGCTATCTG	15600 (16434)
L rev	CCATTCTTTGGTCTCCTTGAC	15746r (16580r)
L-Tr fwd	TGAACTCCGGAACCCTAATC	15791 (16625)
L-Tr rev	AAAGCTGGGAATAGAAACTTCG	15887r (16721r)

^aBinding site are as in the Moraten vaccine strain reference sequence (GenBank accession number AF266287.1) and/or in the MVvac(GFP)H sequence (parentheses) for sequences downstream of the H gene.