FIG 1.
Virus mutants. The relevant regions of the HSV-1 genome are shown. Black arrows represent altered genes. The ΔUL16S and ΔVP22 null mutants were generated by removal of their coding sequences (UL16 and UL49, respectively) from the wild-type BAC.KOS plasmid. The UL16 and VP22 coding sequences were restored to generate ΔUL16Rev and ΔVP22Rev, respectively. For the ΔUL16Rev35 strain, the UL35 coding sequence was replaced with that for UL16 in order to rule out context-specific defects associated with the deletion.