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. Author manuscript; available in PMC: 2014 Apr 1.
Published in final edited form as: Nat Methods. 2013 Jul 25;10(10):973–976. doi: 10.1038/nmeth.2600

Figure 2.

Figure 2

RNA-guided activation of human genes relevant to cell and gene therapy, genetic reprogramming, and regenerative medicine. HEK293T cells were transfected with the dCas9-VP64 expression plasmid and four gRNAs individually or in combination. Target gene expression was measured by qRT-PCR and normalized to GAPDH mRNA levels. Data are shown as the mean ± s.e.m (n = 3 independent experiments). Treatment with the combination of gRNAs was statistically different than all other treatments (*P < 0.05) by Tukey’s test.