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. Author manuscript; available in PMC: 2014 Feb 3.
Published in final edited form as: Dev Biol. 2010 Jun 9;344(2):836–848. doi: 10.1016/j.ydbio.2010.05.514

Figure 4.

Figure 4

Notch inhibition leads to ectopic neuronal differentiation. Transverse section through the ophthalmic trigeminal (opV) placode region of a 30-32 ss embryo. The 13-15 ss heads were cultured in either DMSO or DAPT for 24 hr, harvested, cryosectioned, and immunostained for the opV marker Pax3 (A,D) and the early neuronal marker Islet1 (B,E). DMSO treated embryos showed normal opV placode development (A-C). DAPT treated embryos showed a significant increase in ectodermal Pax3+ cells coexpressing Islet1, the number of Pax3+/Islet1+ mesenchymal cells was also dramatically increased (D-F). Histogram describing total number of cells for Pax3+ ectoderm, Pax3+/Islet1+ ectoderm, Pax3+ mesenchyme, and Pax3+/Islet1+ mesenchyme in DMSO vs. DAPT cultures (F). Error bars depict SEM.