FIG 6.

Expression and localization of Cx43 in AZ-521 and HeLa cells. HeLa cells were transduced with lentiviral particles expressing Cx43 or a control lentivirus, and transduced cells were then selected (designated HeLa-Cx43 or HeLa-control, respectively). AZ-521, HeLa-control, and HeLa-Cx43 cells were examined for expression and localization of Cx43. AZ-521, HeLa-control, and HeLa-Cx43 cells were fixed, permeabilized, and stained to detect Cx43, as described in Materials and Methods. The nucleus is shown in blue, and Cx43 is shown in red. Bars, 10 μm. Cx43 was detected in AZ-521 cells (A) and HeLa-Cx43 cells (C) but not in HeLa-control cells (B).