FIG 3.

SPI-2 effector SseJ is distributed along the membranous system of the infected cell to a lesser extent in fibroblasts than in epithelial cells. Shown are immunofluorescence microscopy images of NRK-49F fibroblasts and HeLa epithelial cells infected with the S. Typhimurium chromosomally tagged strain sseJ::3×FLAG for 24 and 6 h, respectively. The distribution of the endogenous tagged SseJ-3×FLAG protein was examined with anti-FLAG antibodies. Note the distinct pattern between host cell types even with similar numbers of intracellular bacteria. Arrows point to intracellular bacteria located inside the fibroblasts, and the arrowheads point to SseJ present in filamentous membranous structures of HeLa epithelial cells.